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Happy Mother's Day and Cooking in a Dutch Oven
It's finally getting warm enough to get the plants and seeds in the ground. I know many of you already are seeing tomatoes on your plants and here we are just now putting them in the ground. Here in southwestern Idaho, we're right on time (well, maybe a little early with our fingers crossed).
We did more Mother's Day celebrating than gardening this weekend on the homestead. We decided we'd take a trip up to Idaho City, Idaho and drive a short ways up the Ponderosa Pine Scenic Byway (http://en.wikipedia.org/wiki/Idaho_State_Highway_21) and find a spot to cook a picnic in a Dutch Oven. It's good to take a moment for the mammas in our lives (we should do it way more than just the one day a year in May).
If you are wondering about the recipe for the Dutch Oven Pizza we cooked:
Campfire Dutch Oven Pizza
1 1/2 | lbs. lean ground beef | 1/2 | medium red onion; diced |
2 | tsp. italian seasoning | 3 | Tbs. diced greeen bell pepper |
1 | tsp. garlic powder | 3 | Tbs. diced red bell pepper |
salt and black pepper to taste | 1 | (8 oz.) can mushroom stems & pieces; drained | |
2 | Tbs. olive oil | 12 | black olives; sliced |
1 | can crescent rolls | 8 | oz. shredded Cheddar cheese |
1 | jar pizza sauce | 8 | oz. shredded Mozzarella cheese |
Heat a 12" Dutch oven using 18-20 briquettes bottom until hot. In a medium bowl add ground beef, italian seasoning, garlic powder, salt and pepper; mix together with your hands. Drop ground beef by small pieces into the hot Dutch oven and fry until brown. Remove browned beef from Dutch oven and wipe oven down with a paper towel. Pour olive oil into Dutch oven and spread evenly over bottom of oven. Unroll the can of crescent rolls and line the bottom of the oven with a layer of flattened rolls. Spoon pizza sauce evenly over crescent rolls. Sprinkle evenly with seasoned ground beef, red onion, bell peppers, mushrooms, olives, and top with Cheddar and Mozarella cheeses. Cover and bake using 8-10 briquettes bottom and 16-18 briquettes top for 20-30 minutes until crust is browned on edges and cheese is bubbly. Serves: 6-8 This recipe was taken from one of my favorite web sites: http://papadutch.home.comcast.net/~papadutch/dutch-oven-recipes.htm |
LIF mouse embryonic stem cell proliferation and pluripotency under serum free conditions
ReplyDeleteKES mouse embryonic stem cells cultured in serum-free conditions, research LIF in mouse embryonic stem cell proliferation and pluripotency under serum-free conditions. Joint use of small molecules SU5402, PD184352 and CHIR99021 or used alone LIF cultured mouse embryonic stem cells, detected under serum-free conditions LIF on mouse embryonic stem cell self-renewal and pluripotency. Combination of limiting dilution culture the KES cell monoclonal formation rate statistics the monoclonal under different culture systems. The results showed: under serum free conditions, used alone LIF, can not maintain self-renewal and pluripotency of mouse embryonic stem cells; containing three kinds of small molecule drugs SU5402, PD184352, and CHIR99021 (3i) serum-free medium can be maintained in mice embryonic stem cell self-renewal, passaged more than 60 generations still maintain pluripotency; Add LIF 3i culture system significantly improves the mouse embryonic stem cell monoclonal formation rate. Showed that under serum-free conditions, LIF alone can not maintain the pluripotency of embryonic stem cells in mice. 3i culture system can maintain the pluripotency of embryonic stem cells without the need of LIF; 3i conditions, LIF can significantly promote the proliferation of embryonic stem cells in vitro and the monoclonal formed.
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